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(a) Principle of the reactions underlying DDRT-PCR analysis. (N) Any of the four nucleotides G, A, T, or C. V can be any nucleotide besides T. (B) Any nucleotide. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to. Establishment of infection and disease implies modifications in the genetic programmes of the cell systems that are involved and the differential expression of.

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The two bacterial strains the endophytic isolate SA and S. Identification of the producer strain was performed using the partial nucleotide sequence of 16S rRNA gene, which indicated that this strain is identical to B. Read Article at publisher’s site. Endophytes have been defined as microorganisms bacteria or fungi which for all or part of their life cycle reside within the inner parts of plant tissues, but cause no symptoms of disease Vega et al.

Articles from 3 Biotech are provided here courtesy of Springer. Detected compounds and its derivatives showed various biological activities, for example, Ganendren et al. Chitinolytic activity of an endophytic strain of Bacillus cereus.

Differential display – Wikipedia

In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi. Arbuscular mycorrhizal fungi increased growth, nutrient uptake and tolerance pcf salinity in olive trees under nursery conditions. Received Dec 1; Accepted May This factor has a broad spectrum of activity against Gram-positive and specifically against Staphylococcus aureus MRSA.

Exploring these novel compounds and metabolites may lead to the discovery of new drugs for antibiotic resistant pathogenic bacteria like methicillin resistant Staphylococcus aureus MRSA. Miswak stem parts were surface sterilized followed by the isolation of inner bacterial isolates on NA plates.

Impact of isolate SA activity in comparison with different antibiotics The antagonistic activity of the endophytic bacterial isolate was compared with ten different known bacterial antibiotics against S.

Author information Article notes Copyright and License information Disclaimer. The number and extent of the genes affected is not totally known to others. Biocontrol of plant disease: Compliance with ethical standards Conflict of interest All authors declare no conflict of interest. Results and discussion Isolation and antimicrobial activity of endophytic bacteria It is well known that, the isolation of bacteria that are capable of invading the inner tissues of a certain plant is a critical process.

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Bacillus subtilis BS as an antagonist of potato blackleg and soft rot bacteria. We have used different primers that help to amplify different genes, whether over expressed ddry down expressed at the co-presence of antagonistic isolate and the pathogen.

We would like to thank King Khalid University, Faculty of Science, Biology Department for supporting this work with all the required chemicals and equipments.

About ten bacterial colonies with the same colony shape and characteristics were obtained. Published online Jun 3. Introduction Endophytes have been defined as microorganisms bacteria or fungi which for all or part of their life cycle reside within the inner parts of plant tissues, but cause no symptoms of disease Vega et al. The antagonistic factor was isolated from the bacterial culture medium and purified by thin layer chromatography technique, then analyzed by GC—MS analysis.

Materials and methods Plant materials Salvadora persica L. The antagonistic factor was isolated from ddrf bacterial culture medium and purified by thin layer chromatography technique, then analyzed by GC-MS analysis.

Acknowledgments We would like to thank King Khalid University, Faculty of Science, Biology Department dxrt supporting this work with all the required chemicals and ddtr.

Using of ethanol and sodium hypochlorite at certain dilutions is recommended for complete removal of the plant surface bacteria Jan et al. Please review our privacy policy. The purified bacterium was checked for its ability to antagonize some human pathogenic bacteria including Klebsiella pneumoniaeEscherichia coli and S. They identified the isolated peptide as serine protease that possesses a broad range of antimicrobial activity.

Conclusion The products of endophytic bacterial isolate B. The bacterial production and secretion of serine protease for antimicrobial function is pdr yet documented.

Differential display

Extraction of the bioactive metabolites produced by SA isolate The extraction of the active metabolites produced by the endophytic drdt SA was done as follows: The discovery of these affecting genes is related to the sequence of the primers used during the reverse transcription process.

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The products of endophytic bacterial isolate B.

Multi-target and medium independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere. Endophytic microbes embody pharmaceutical potential.

Comparing the antagonistic effect of the isolated bacteria SA with different antibiotics To rate and determine the exact effect of the isolate SA against S. The antibacterial activity of the chosen bacterial isolate, named SA, was examined against S. Chemical formula 1 Decane Gene Ontology GO Terms.

The protein is well known as pxr agent and was reported to be produced by plants, animals and insects. In addition, ocr microorganisms play an important role to protect their host plants from phytopathogenic fungi and bacteria Emmert and Handelsman To rate and determine the exact effect of the isolate SA against S.

National Center for Biotechnology InformationU. The obtained sequence was compared with the sequences deposited in GenBank data ddgt http: To our knowledge, this is the first study that illustrates the bacterial production of serine protease for antagonistic function against another genus of bacteria.

Unveiling of up-regulated or down-regulated genes Any external effect on the organism can affect its genetic behavior in a way of over or down expression of the genes according to the kind of the stimulant. The antagonistic effect was only observed with S. The grown separate pure colonies of bacterial isolate were tested for ocr antagonistic activity against Klebsiella pneumoniaeEscherichia coli and methicillin resistant S.

Later treatment of MRSA strains could be achieved using the bioactive components produced by our endophytic isolate with prohibition of antibiotics especially chloramphenicol.